USE OF MUELLER-HINTON BROTH AND AGAR IN THE GERM TUBE TEST
نویسندگان
چکیده
منابع مشابه
Use of Mueller-hinton Broth and Agar in the Germ Tube Test
Candida albicans is often isolated from clinical samples, thus its presumptive differentiation from other species of the same genus can be based on its ability to form the germ tube in human serum. Nevertheless, there are two other species that share this characteristic: C. dubliniensis and C. africana. The aim of this study was to compare four different substrates to perform the germ tube (GT)...
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A collaborative study was undertaken to evaluate the performance of currently marketed Mueller-Hinton agars from seven manufacturers by replicate disk diffusion tests with standard quality control strains. Identification of the manufacturers was concealed, and the resulting data were evaluated for the selection of a physical reagent standard against which the performance of future production lo...
متن کاملEvaluation of Mueller-Hinton agar for disk diffusion susceptibility tests.
Twelve lots of commercially prepared Mueller-Hinton agar from four medium manufacturers were evaluated for performance with recommended quality control organisms, medium pH, agar depth and uniformity, and characteristics of the zones of inhibition. Only 2 of 12 lots were acceptable for disk diffusion susceptibility tests. Significant problems were observed with the preparation and pouring of th...
متن کاملComparison of cation-adjusted Mueller-Hinton broth with Iso-Sensitest broth for the NCCLS broth microdilution method.
Comparison of MIC results obtained in different parts of the world is currently difficult because of variations in methods. In this study, cation-adjusted Mueller-Hinton broth, the NCCLS-recommended medium, was compared with Iso-Sensitest broth, which is widely used in Europe. Microbroth dilution testing, using the NCCLS procedure, was performed on 124 Gram-positive (staphylococci and enterococ...
متن کاملMueller-Hinton broth undergoes visible oxidative color changes in the presence of peroxidase and hydrogen peroxide.
In the presence of peroxidase and hydrogen peroxide, Mueller-Hinton broth undergoes a slow but clearly detectable color change from pale yellow to dark yellow or brown. An investigation of this phenomenon led to the conclusion that it is the result of the oxidation of tyrosine, a major component of the broth. Indeed, tyrosine has long been known to oxidize upon treatment with peroxidase and hyd...
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ژورنال
عنوان ژورنال: Revista do Instituto de Medicina Tropical de São Paulo
سال: 2014
ISSN: 0036-4665
DOI: 10.1590/s0036-46652014000600005